Low-cost automated pipetting system using a single board computer and 3D-printing.

Autor: Boppana, Naga P. D., Snow, Robyn A., Simone, Paul S., Emmert, Gary L., Brown, Michael A.
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Zdroj: Instrumentation Science & Technology; 2023, Vol. 51 Issue 4, p355-370, 16p
Abstrakt: A low-cost, automated pipetting system (auto pipet) was developed to deliver microliter volumes of aqueous solutions. This system was constructed using a single board computer, 3 D-printed parts, stepper motors, and other commercially available components. The performance of the auto pipet was found to be comparable or better than manual pipetting and a commercially available liquid handling system. Inter-day, intra-day, inter-instrumental, and intra-instrumental validation studies were performed to evaluate the accuracy and precision of liquid delivery using the auto pipet. Accuracy was determined to be from 98 to 102% with the precision, represented as the relative standard deviation, to be less than 3%. The auto pipet was further evaluated by the preparation of calibration and check standards for the determination of method detection limit, accuracy, and precision for trihalomethanes using a commercially available monitoring system. The auto pipet was also used as a digital buret to perform water hardness and water alkalinity titrations with promising results. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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