| Autor: |
Godoi-Jr, Ederaldo P., Bronzato, Juliana D., Francisco, Priscila A., Bícego-Pereira, Eloá C., Lopes, Erica M., Passini, Maicon R. Z., de-Jesus-Soares, Adriana, Almeida, José F. A., Marciano, Marina A., Ferraz, Caio C. R., Gomes, Brenda P. F. A. |
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| Zdroj: |
Clinical Oral Investigations; May2023, Vol. 27 Issue 5, p2049-2064, 16p, 1 Diagram, 6 Charts, 2 Graphs |
| Abstrakt: |
Objective: The objective of this study was to evaluate the microbiological profile of root canals indicated for endodontic retreatment due to secondary endodontic infections evidenced by the presence of chronic apical periodontitis (G1) or for prosthetic reasons, without clinical and radiographic signs of endodontic reinfection (G2). Methods: Microbiological samples were collected from thirty teeth (N=30) out of which 15 were indicated for retreatment due to the presence of chronic apical periodontitis (G1) and 15 were indicated exclusively for prosthetic reasons (G2). Samples were collected from root canals before (S1), after chemomechanical preparation (S2), and after 30 days of intracanal medication composed of calcium hydroxide and 2% chlorhexidine gel (S3). The molecular analysis was performed using Nested-PCR for the detection of 17 bacterial species. The efficacy of each stage of the retreatment in reducing the microbial load was verified by counting colony-forming units (CFU). The statistical analysis considered a significance level of 5%. Results: The results showed that bacteria were detected in 100% of the cases in S1, in both groups, by Nested PCR. The most frequently found species in S1 in both groups were Enterococcus faecalis, Fusobacterium nucleatum, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Parvimonas micra. The microbial load of G1 was higher than G2 in the initial samples (S1). Endodontic retreatment was effective in reducing the microbial load in G1 and G2. Statistically significant associations were found between specific bacteria and clinical signs and symptoms. Conclusion: It was concluded that the microbial community present in the root canal of teeth indicated for endodontic retreatment is mixed and heterogeneous. G1 and G2 differ in the number of species per case and microbial load. Clinical relevance: Endodontic retreatment was effective in reducing the microbial load. Statistically significant associations were found between specific bacteria and clinical signs and symptoms. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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