| Autor: |
Rumende, Rooije R. H., Rares, Fredine E. S., Rares, Laya M. |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2023, Vol. 2694 Issue 1, p1-13, 13p |
| Abstrakt: |
Percoll density gradient centrifugation (SGDP) method is widely used for sexing spermatozoa, but recent research results show that the SGDP method causes damage to spermatozoa membranes which results in a decrease in sperm quality. The main objective of this study was to analyze the sperm membrane integrity of the administration of PC phospholipids (phosphatidylcholine)+EGTA Ca2+ free buffer, the quality of spermatozoa cattle after SGDP process. The sample used is semen derived from cows male Frishian Holstein (FH) aged 5-8 years. The research used experimental laboratory study methods. The observed variables were: spermatozoa membrane integrity, spermatozoa motility, spermatozoa viability, uncapacitated spermatozoa, capacitated spermatozoa and spermatozoa that underwent acrosomal reaction. The results showed that the administration of phospholipid PC (phosphatidylcholine)+EGTA Ca2+ free buffer makes spermatozoa membrane integrity gave the highest effect on spermatozoa that had not undergone capacitation of 0.93, spermatozoa that underwent acrosomal reaction of 0.879, and spermatozoa viability of 0.814. The results of the model test showed that the effect of membrane integrity on spermatozoa undergoing acrosomal reactions was very significant (p<0.01), meaning that if membrane integrity increased by 1%, it would increase spermatozoa undergoing acrosomal reactions by 0.9025. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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