Pre-Protection and Mechanism of Crude Extracts from Dioscorea alata L. on H 2 O 2 -Induced IPEC-J2 Cells Oxidative Damage.

Autor: Yun, Yanhong, Shi, Huiyu, Wang, Yanyu, Yang, Fengyuan, Zhang, Yuanxin, Feng, Haibo, Chen, Junpu, Wang, Xuemei
Předmět:
Zdroj: Animals (2076-2615); Apr2023, Vol. 13 Issue 8, p1401, 18p
Abstrakt: Simple Summary: The Dioscorea alata L. is known to contain abundant proanthocyanidins and anthocyanins, which exhibit potent antioxidant properties capable of scavenging free radicals. Additionally, these compounds have been shown to effectively promote the proliferation of IPEC-J2 cells within a pharmacotoxicity range. Conversely, exposure to H2O2 can damage IPEC-J2 cells and trigger increased activity of cellular antioxidant enzymes. This study investigated the impact of crude extract from Dioscorea alata L. on the antioxidant capacity of IPEC-J2 cells and explored the pre-protective mechanism of Dioscorea alata L. against oxidative damage induced by H2O2 through an examination of its effect on the NF-κB inflammatory signaling pathway. The purple tubers of Dioscorea alata L. have been found to contain a variety of bioactive chemical components, including anthocyanins, which make it significant to investigate the pre-protective effects of Dioscorea alata L. and its crude extracts on cells prior to oxidative stress. To establish a suitable oxidative damage model, an injured model of IPEC-J2 cells was created using H2O2 as the oxidant. Specifically, when the concentration of H2O2 was 120 μmol/L and the injured time was 8 h, the survival rate of cells decreased to approximately 70%, and the cells exhibited a noticeable oxidative stress reaction. Moreover, the crude extracts of Dioscorea alata L. demonstrated beneficial pre-protective effects on IPEC-J2 cells by increasing the total antioxidant capacity (T-AOC) and catalase (CAT) activities, augmenting the expression of total superoxide dismutase (T-SOD) and its genes, reducing the content of malondialdehyde (MDA) and the activity of glutathione peroxidase (GSH-PX) and its expression of genes, and promoting the expression of glucose transporter SGLT1 gene while reducing that of GULT2 gene, thereby facilitating the entry of anthocyanins into cells. In addition, the 50 μg/mL crude extracts effectively inhibited the phosphorylation of IκB and the p65 protein, thus reducing cellular oxidative stress. Given these findings, Dioscorea alata L. can be considered a natural antioxidant for practical breeding and production purposes, with an optimal concentration of crude extracts in this experiment being 50 μg/mL. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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