| Autor: |
Lai, C. K. W., Ho, S. S., Chan, C. H. S., Leung, R., Lai, K. N. |
| Předmět: |
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| Zdroj: |
Clinical & Experimental Allergy; Feb1996, Vol. 26 Issue 2, p138-146, 9p |
| Abstrakt: |
Background Interleukin (IL)-3 and granulocyte macrophage colony-stimulating factor (GM-CSF) may influence the inflammatory process in asthma through their regulatory role on eosinophil survival, differentiation and effector function. Objective To examine the relationships between IL-3 and GM-CSF messenger (m) ribonucleic acid (RNA) expression in peripheral blood CD4+ cells and serum levels of eosinophil cationic protein (ECP). a marker of eosinophil activation, and disease activity in asthma. Methods Venous blood was drawn from patients with acute severe asthma prior to the commencement of systemic steroid therapy (day 1) and 7 days afterwards (day 7), patients with stable disease and normal healthy volunteers. The capacity for expression of IL-3 and GM-CSF in ex vivo stimulated circulating CD4+ cells was assessed semi-quantitatively by reverse transcription-polymerase chain reaction (RT-PCR). Results We found that the capacity for expression of IL-3 and GM-CSF was significantly higher in acute asthmatics prior to steroid treatment (n = 24) than those in stable disease (n = 38) and healthy subjects (n = 32, P< 0.001 for IL-3 and <0.05 for GM-CSF), but no difference was observed between the latter two groups. Further assessment made in 15 of the 24 acute asthmatics 7 days after systemic steroid treatment revealed a significant reduction in GM-CSF expression (P<0.05) but not for IL-3. At the same time. PEF also improved significantly from 30.4±3.5% of predicted value to 72.9±7.2% (P<0.0001) and serum ECP concentration also fell from l9.9±5.9 μg/L to 4.3±2.0 μg/L (n= 10, P<0.01). Conclusion Our data suggest both IL-3 and GM-CSF may be important in the pathogenesis of acute severe asthma. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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