| Autor: |
SAAD, S., KECHEBAR, M. S. A., KAROUNE, S., LAHMADI, S., DJEMOUAI, N., FOUGHALIA, A., SIABDALLAH, N., DJEDIDI, M., ABIDAT, H. |
| Předmět: |
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| Zdroj: |
Applied Ecology & Environmental Research; 2023, Vol. 21 Issue 2, p889-923, 35p |
| Abstrakt: |
Despite the great therapeutic and industrial values of Lepidium sativum L., all scientific reports have mainly focused on studying its seeds. For the first time, the aim of this study is to determine the effects of extraction methods and solvents on the phenolic contents and the antioxidant capacity of Lepidium sativum L. aerial parts and roots. Different techniques such as maceration, Soxhlet and ultrasound-assisted extraction were applied. The antioxidant effects were examined by four tests; 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), reducing power and phenanthroline assays. The greatest content of total phenolic content was obtained for the methanolic fraction of aerial parts prepared by maceration while the chloroformic fraction of roots extracted by the Soxhlet gave the highest content of total flavonoids. L. sativum fractions demonstrated good antiradical and chelating activities. High-performance liquid chromatography (HPLC) profiles revealed the presence of flavonoids, phenolic acids and other phenolic compounds. However, the fraction obtained by Soxhlet was the richest in bioactive compounds compared to that prepared by maceration. Multivariate analysis showed that L. sativum fractions belong to three distinct types in terms of their polyphenolic content and antioxidant activity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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