| Autor: |
Chang, Z. L., Hoffman, T., Bionvini, E., Stevenson, H. C., Herberman, R. B. |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology; Nov1983, Vol. 18 Issue 5, p439-449, 11p |
| Abstrakt: |
Human peripheral blood mononuclear cells (PBMNC) wore found lo be cytotoxic for mouse or human anchorage-dependent target cell lines in a 48-72 h [125I]iododeoxyuritline (IUOR) release assay. Unfractionated, adherent or nonadherent cells had significant levels of cytoioxicity, as did cells fractionated according to size into 'lymphocytes' or 'monocytes' by elutriation. Intermediate size cells, not enriched for monocytes, had high levels of cytotoxicity. In all fractions tested, including adherent populations, some cells with the morphology of large granular cells were observed. Treatment of all fractions with interferon (IFLrA, a purified, recombinant α-IFN) boosted cytotoxicity against four target cells lines. Treatment with lymphokines containing putative 'macrophage-activating factor' (MAF) also enhanced cytotoxicity in fractions depleted of monocytes. Culture in fetal bovine serum enhanced cytotoxicity mainly in unfractionated and nonodherent PBMNC. These experiments indicated that N K-like cells can be appreciable contaminants in elutriator-purified monocyte-enriched or adherent cell populations and thereby contribute to observed cytotoxicity, particularly after pretreatment with IFN or other stimulatory factors. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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