| Autor: |
Endoh, Keita, Hanaoka, So, Matsushita, Michinari, Ubukata, Masatoshi, Yamada, Hiroo |
| Předmět: |
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| Zdroj: |
New Forests; May2023, Vol. 54 Issue 3, p515-523, 9p |
| Abstrakt: |
The cryopreservation of organs, tissues and cells is a useful tool for long-term, ex situ conservation of forest tree species. Since Betula platyphylla, B. ermanii and B. maximowicziana are economically and ecologically important forestry trees in boreal areas of Japan, meaningful conservation efforts require the development of cryopreservation methods for the winter buds of Japanese birch genetic resources. This study investigated the cryopreservation of winter buds of Japanese birch by slow freezing. Regrowth rates of shoot primordia isolated from winter buds that were cryopreserved in liquid nitrogen (− 196 °C) after slow freezing from − 2 to − 40 °C at a rate of 0.2 °C/min were comparable with regrowth rates of shoot primordia that were not subjected to slow freezing and cryopreservation. Isolated shoot primordia of B. platyphylla showed regrowth rates of approximately 80%, and the regrowth rates of isolated shoot primordia of B. ermanii and B. maximowicziana were approximately 30%. Prevention of fungal contamination was technically difficult when preparing tissue cultures from B. ermanii and B. maximowicziana buds under sterile conditions. Assessments of freezing adaptation by differential thermal analysis showed that birch buds responded to subzero temperatures by extracellular freezing, which is a common mechanism for winter freezing adaptation in many plant tissues, but is different from deep supercooling associated with extraorgan freezing observed in the winter buds of many tree species. Since birch buds could tolerate immersion in liquid nitrogen by extracellular freezing, slow freezing of winter buds is considered to be a feasible method for preparing birch buds for cryopreservation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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