| Autor: |
Paul-Eugene, N., Dugas, B., Gordon, J., Kolb, J. P., Cairns, J. A., Paubert-Braquet, M., Mencia-Huerta, J. M., Braquet, P. |
| Předmět: |
|
| Zdroj: |
Clinical & Experimental Allergy; Apr1993, Vol. 23 Issue 4, p317-325, 9p |
| Abstrakt: |
The effect of β2-adrenoceptor agonists and interleukin-4 (IL-4) on the CD23 expression on, and release from, the human promonocytic cell line, U 937, was investigated. As assessed by flow cytometry, incubation of U 937 cells in the presence of salbutamol, fenoterol or IL-4 induced a concentration- and time-dependent increase in CD23 expression, that was maximal after 48 hr and followed by a decrease thereafter. In addition, salbutamol potentiated the effect of IL-4, the optimal concentration of the drug being a function of the concentration of this cytokine. This synergy between IL-4 and β2-adrenoceptor agonists was also observed for the release of the soluble form of CD23. The effect on CD23 expression of salbutamol and fenoterol, but not of IL-4, was blocked in the presence of D,L-propranolol (1 μM) or butoxamine (1 μM). The α- adrenoceptor agonist, norepinephrine (1 μM), was ineffective in inducing CD23 expression or potentiating the one evoked by IL-4. Salbutamol down-regulated the expression of FcγRI (CD64) and FcγRII (CD32) whereas IL-4 was ineffective. Only when added together at the onset of the culture did salbutamol and IL-4 induce, after 48 hr, the expression of the monocyte marker, CD14. The expression of CD18 was up- regulated in response to salbutamol either alone or in combination with IL-4, this cytokine alone being inefficient. These data suggest that IL-4 and β2-adrenoceptor agonists induce differentiation of U 937 cells into monocyte-like cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
