Vascular Endothelial Growth Factor Single Nucleotide Polymorphism +405 G/C (rs2010963) is associated with Levels, Infection Severity, and Amputation among South Indian Diabetic Foot Ulcer Patients.

Autor:	Ganapathy, Priyanka, Devanatha Desikan Sheshadri, Vidya, Sarkar, Rajesh, Jones, Sumathi, Gunasekaran, Krishnamoorthy, Teka, Feyisa Obsa, Umapathy, Dhamodharan, Basha, Saleem
Předmět:	DIABETIC foot SINGLE nucleotide polymorphisms INFECTION SEVERITY of illness index COMPARATIVE studies PSYCHOSOCIAL factors GENOTYPES ENZYME-linked immunosorbent assay VASCULAR endothelial growth factors AMPUTATION POLYMERASE chain reaction PEOPLE with diabetes
Zdroj:	Evidence-based Complementary & Alternative Medicine (eCAM); 4/13/2023, p1-10, 10p, 1 Black and White Photograph, 5 Charts, 3 Graphs
Abstrakt:	Background. The regulation of vascular endothelial growth factor (VEGF) by genetic factors in T2DM and DFU still requires thorough investigation. Hence, the present study aimed to investigate the association of VEGF +405 G/C in DFU subjects and correlate it with its circulatory levels, infection severity, and amputation rate. Materials and Methods. This study registered a total of 754 participants of which group I: healthy controls (n = 297), group II: T2DM subjects (n = 242), and group III: DFU subjects (n = 215). Genotyping and levels of rs2010963 were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and ELISA, respectively. Results. Results of the current study showed a clear decline in circulatory VEGF-A levels in DFU subjects. VEGF-A was decreased in DFU subjects with the mutant "CC" genotype. The mutant "CC" of VEGF +405G/C was also found to be more susceptible to ulcer grade (III and IV) and major amputations. Conclusion. VEGF +405G/C SNP is associated with levels, infection severity, and amputation amongst South Indian DFU patients. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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