| Autor: |
Stone, G. L., Mshana, R. N., Touw, J., Belehu, A. |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology; Jan1982, Vol. 15 Issue 1, p33-48, 16p |
| Abstrakt: |
Sixteen healthy siblings were identified as HLA-D-identical to 12 borderline lepromatous or polar lepromatous leprosy patients by the absence of a mixed lymphocyte reaction (MLR). The peripheral blood mononuclear cells (PBM) of the healthy siblings showed a lymphoproliferative response (Δcpm) to Mycobacterium leprae antigens which was about fivefold or more greater than that of the lepromatous patients. Lepromatous PBM, with or without mitomycin C treatment, were co-cultured with a constant number of normal PBM. In other experiments the two cell types were co-cultured in various proportions, with the total cell number kept constant. Neither approach resealed suppressor cells in lepromatous PBM capable of suppressing the lymphoproliferative response to M. leprae. On the contrary, we found that leprornatous PBM can respond to M. leprae antigens if the sensitized lymphocyte is provided by mitomycin-C-treated normal PBM. Additionally, experiments in which isolated adherent cells and non-adherent cells of sibling pairs were recombined Failed to reveal a detect in the M. leprae antigen-presenting function of lepromatous adherent cells, Since we found no evidence that sensitized cells are present in lepromatous PBM with their function unexpressed (due to a monocyte defect) or suppressed (due to suppressor cells), we conclude that lepromatous patients simply lack sufficient numbers of antigen-specific T lymphocytes to initiate a lymphoproliferative response to M. leprar antigens. The reason for their absence remains an important unanswered question. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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