| Abstrakt: |
Objective To analyze the expression of immune cells and immunosuppressive factors in the immune microenvironment of glioblastoma. Methods A total of 30 glioma specimens, all of which were glioblastoma (IDH - wildtype), were surgically removed and completely preserved in the First Affiliated Hospital of Ji'nan University and Guangdong Sanjiu Brain Hospital from November 2020 to April 2021. T lymphocytes (CD3+ T cells, CD4+ T cells, CD8+ T cells), suppressive immune cells [FoxP3+ regulatory T cell (Treg)], immunosuppressive factors [transforming growth factor - β (TGF - β)], immunosuppressive factors [programmed cell death protein ligand 1 (PDL1)] in the immune microenvironment of glioblastoma were detected by immunohistochemistry. Results The proportion of CD4+ T cells in glioblastoma was very low. CD3+ T cells accounted for more than 3% in 15 cases (50%), and CD8+ T cells were similar to CD3+ T cells. Few FoxP3+ Treg cells were detected in only 2 cases (6.67%). The cytoplasm of 24 cases (80%) of glioblastoma showed strong positive expression of TGF - β, and its expression was correlated with the distribution of CD3+ T cells. No CD3+ T cells were found in the areas with high expression of TGF-β, and CD3+ T cells were widely distributed in the areas with low expression of TGF-β. PDL1 expression was non- detected. Conclusions TGF - β protein was highly expressed in glioblastoma, and future drugs or immunotherapies designed to target TGF-β may contribute to the clinical treatment of glioblastoma. [ABSTRACT FROM AUTHOR] |
