| Autor: |
Govind, V., Babu, R. Narendra, Apparao, V., Sriram, P., Kumar, T. M. A. Senthil, Abraham, Robinson J. J. |
| Předmět: |
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| Zdroj: |
Asian Journal of Dairy & Food Research; Mar2023, Vol. 42 Issue 1, p31-37, 7p |
| Abstrakt: |
Background: Enrofloxacin used considerably on large scale among poultry birds. Detection and monitoring of its residues is vital in the domestic and export market. A simple and rapid LCMS/MS method for screening of Enrofloxacin and its metabolite residues in poultry liver samples was described. Methods: Target analytes were extracted with a solvent combination of 5% phosphoric acid in 50% acetonitrile in water. It was followed by deep freezing for efficient separation of the liquid phase. Later extractants were subjected to centrifugation at 4°C, 10,000 rpm for 20 minutes and supernatant was filtered and injected into LCMS/MS. 0.1% formic acid is used as mobile phase additive for both aqueous and organic mobile phase acetonitrile. Result: Accuracy and precision values of the method are within the acceptable limit of (<5% RSD) accorded by the European commission. Standardized method has been applied to determine the Enrofloxacin and Ciprofloxacin residues in liver samples collected in local markets and found within maximum residue limits. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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