| Autor: |
Sudalaimani, S., Arun, S., Esokkiya, A., Sanjeev Kumar, K., Sivakumar, C., Giribabu, K. |
| Předmět: |
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| Zdroj: |
Analyst; 4/7/2023, Vol. 148 Issue 7, p1451-1459, 9p |
| Abstrakt: |
Sensing acetylcholine has been predominantly based on enzymatic strategies using acetylcholine esterase and choline oxidase because of its electrochemical inertness. Electrified liquid–liquid interfaces are not limited to oxidation/reduction processes, and can be utilized to detect non-redox molecules which cannot be detected using conventional solid electrodes. In this study, a disposable micropipette tip based liquid–organogel interface, in the presence/absence of calixarene has been developed as a platform for sensing acetylcholine. We also explored a liquid–liquid interface approach for sensing acetylcholine using a pre-pulled glass micropipette. In both approaches, the configuration, i.e., liquid–organogel and liquid–liquid interface-current linearly increases during the backward transfer of acetylcholine. The simple and facilitated ion transfer of acetylcholine across the liquid–organogel exhibited a linear range of 10–50 μM and 1–30 μM with a detection limit of 0.18 μM and 0.23 μM and a sensitivity of 9.52 nA μM−1 and 9.20 nA μM−1, respectively. Whereas, the detection limit of simple and facilitated ion transfer of liquid–liquid interface using pre-pulled glass micropipette was found to be 0.42 μM and 0.13 μM with a sensitivity of 5 × 10−3 nA μM−1 and 3.39 × 10−2 nA μM−1. The results indicate that the liquid–organogel configuration supported on a disposable micropipette tip without any pre-fabrication is highly suitable for electrified soft interface sensing applications. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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