| Abstrakt: |
Profuse flowering was noticed in 68 sugarcane genotypes during the 2017 and 2018 cropping seasons. To identify location-specific male and female parents, pollens from the anthesized genotypes were collected at 5.30, 6.30, and 7.30 a.m. The viability was tested using potassium iodide (KI) and acetocarmine staining methods. The analysis of variance indicated a significant effect for genotype, time of pollen collection, year, staining methods, and interaction between genotype and time of pollen collection, and staining methods. The pollen viability was high in the early morning collections (5.30 a.m.). The KI stain displayed higher viability percentages than acetocarmine. The pooled mean and 't' test-based analysis of pollen germination revealed that the clone G 2012091 had the highest germination percentages of 75.80 and 77.30 in the acetocarmine and KI staining methods, respectively, while the genotype CoG 93076 had the lowest germination in all three evaluated times. Based on the pollen viability and germination studies, the genotypes G 2012091, G 2012061, G 2012035, G 2014036, and G2012115 were designated as male while CoG 93076 was earmarked as female. To formulate breeding strategies, the effects of crossing and selfing were estimated. In the bi-parental crosses, the fluff germination ranged from 5.4 to 20.80% and from 5.0% to 21.40% during the 2020 and 2021 seasons, respectively, while a decline was noticed in all the selfed seeds. Selfing did not affect quality traits; however, it reduced the performance of quantitative traits. It is ascertained that bi-parental hybridization followed by selection would be rewarding. [ABSTRACT FROM AUTHOR] |
Full text from SpringerLink