| Autor: |
Schlüter, Philipp M., Stuessy, Tod F., Paulus, Hannes F. |
| Zdroj: |
Taxon; Aug2005, Vol. 54 Issue 3, p766-770, 5p, 1 Chart |
| Abstrakt: |
In many plant groups, the use of low-copy nuclear sequence markers for phylogenetics and population genetics has been hindered by their limited availability. Although it may be possible to PCR amplify low-copy markers using primers designed for use with other plant groups, this does not always yield the desired results. Here, we suggest several alternative approaches to begin the isolation and characterisation of novel low-copy markers when there is little or no sequence information available. These alternatives are: (1) the design of new primers from information in the sequence databases; (2) isolation of homologous DNA using a gene probe from another organism; (3) characterisation of sequence markers from DNA fingerprints; and (4) obtaining novel sequences via cDNA cloning. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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