| Autor: |
Kostova, Nora, Staynova, Albena, Popova-Hadjiiska, Ljubomira, Georgieva, Dimka, Ivanova, Ilonka, Aneva, Nevena, Atanasova, Margarita, Hristova, Rositsa |
| Předmět: |
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| Zdroj: |
Journal of Radiation Research; Mar2023, Vol. 64 Issue 2, p471-479, 9p |
| Abstrakt: |
The purpose of the present study is to evaluate the effect of curcumin as a natural compound against radiation induced γ-foci and stable chromosome aberrations. Whole blood samples form three human volunteers were pretreated with curcumin at different concentrations (0.5, 10, 20 and 100 μg/ml). After 1-hour incubation, the lymphocytes were exposed to γ-rays (0.05, 0.5, 1 and 2 Gy). Radiation induced changes in cells were quantified using γ-H2AX/53BP1 assay and FISH analysis. Our results have shown that curcumin significantly reduced the frequency of both γ-foci and translocations. We found concentration-dependent increase of curcumin protective effect on γ-H2AX/53BP1 foci formation at all radiation doses. Concerning the translocations, after 0.05 and 0.5 Gy γ-rays the values of genomic frequencies are comparable within each dose and we did not observe any impact of curcumin. The most protective effect after 1 Gy exposure was found at 100 μg/ml curcumin. At 2 Gy irradiation, the maximum protection was achieved at 0.5 and 10 μg/ml of curcumin. Concentrations of 20 and 100 μg/ml also prevent lymphocytes but to less extent. Our in vitro study indicates radioprotective efficacy of curcumin against γ-ray induced damages in human lymphocytes. This observation suggests that curcumin may play a role to protect patients undergoing radiological procedures. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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