| Autor: |
Junyi Xu, Xin Li, Jinglian Bai, Ying Liu, Shaojie Wang, Yueting Liu, Chunguang Yang |
| Předmět: |
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| Zdroj: |
Oil Crop Science; Oct2022, Vol. 7 Issue 4, p180-188, 9p |
| Abstrakt: |
The main advantage of digital PCR (dPCR) is that it facilitates absolute quantification of the target without reference to the standard/calibration curve. Crystal droplet dPCR has a three-color staining detection function, which enables multiplex PCR reaction. In this study, this technique was used to establish triple dPCR detection for the genetically modified soybean MON87708 x MON89788 with stacked traits. Specific absolute quantitative detection was accomplished for the genomic DNA extracted from the homogenized seeds of GM stack MON87708 x MON89788 soybean. Our results can serve as a reference for the absolute quantitative detection of stacked events of genetically modified crops. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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