Immunolocalization of homogalacturonans in the apex of the long-day plantSinapis albaat floral transition. The pectin content drops dramatically in the first hours of this transition.

Autor:	Sobry, Stéphanie, Havelange, Andrée, Liners, Françoise, van Cutsem, Pierre
Předmět:	MERISTEMS PLANT growth PLANT physiology ELECTRON microscopy IMMUNOGLOBULINS PLANT cells & tissues
Zdroj:	Physiologia Plantarum; Mar2005, Vol. 123 Issue 3, p339-347, 9p
Abstrakt:	The pectin content in the meristem ofSinapis albaat flowering transition was assessed in electron microscopy using immunocytochemistry and the monoclonal antibody 2F4 that recognizes a Ca2+-induced supramolecular conformation of homogalacturonans (Liners et al. Plant Physiol 91: 1419–1424, 1989; Liners et al. Plant Physiol 99: 1099–1104, 1992). The meristem's pectins were not recognized at all by the 2F4 monoclonal antibody unless they were enzymatically (PME: pectin methylesterase) or chemically (NaOH) de-esterified, indicating that native pectin must be largely esterified in the apical meristem. A striking but transient decrease of the homopolygalacturonic content of the meristem's cell walls occurred between 20 and 24 h after start of the inductive long day. These changes suggest a role for pectin in floral transition. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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