| Autor: |
Peng Dong, Lai Yujian, Tian Jianxin, Zhong Biluan, An Miaoqing, Li Pan, Du Bing |
| Předmět: |
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| Zdroj: |
Journal of Chinese Institute of Food Science & Technology / Zhongguo Shipin Xuebao; Feb2023, Vol. 23 Issue 2, p72-82, 11p |
| Abstrakt: |
The study aimed to investigate the mechanism of the peptide from yellow croaker swim bladder inducing apoptosis in prostate cancer DU-145 cells. Isolation and purification of the peptides from the enzymatic hydrolysate of yellow croaker swim bladder and identification of its amino acid sequences. The CCK-8 method was used to measure the inhibition rate of swim bladder peptide after treatment with YCSB-lc. AO/EB method was used to determine apoptosis in DU-145 cells. Flow cytometry was used to determine apoptosis rate and cell cycle in DU-145 cells, and western blot was used to determine the expression levels of apoptosis-related proteins. The results showed that the YCSB-lc contains two oligopeptides, Ser-Pro-Ser-Pro and Gly-Pro-Ala-Arg. Compared with the control group, the apoptotic cells in the YCSB-lc groups were significantly increased in a dose-dependent manner. Flow cytometry results showed that live cells in YCSB-lc groups were significantly decreased, while the late apoptotic cells were significantly increased. YCSB-lc blocked the cell cycle of DU-145 cells in G0/G1 phase, up-regulated the protein expression of Bax, Caspase-3 and Caspase-9, and induced DU-145 cells apoptosis. YCSB-lc could effectively induce apoptosis in DU-145 cells, and the underlying mechanism is related to cell cycle block and mitochondria-mediated apoptotic pathway. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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