Autor: |
Fernández-Mateo, Raúl, Calero, Víctor, García-Sánchez, Pablo, Ramos, Antonio, Morgan, Hywel |
Zdroj: |
Microfluidics & Nanofluidics; Mar2023, Vol. 27 Issue 3, p1-7, 7p |
Abstrakt: |
We describe a user-friendly, open source software for single-particle detection/counting in a continuous-flow. The tool automatically processes video images of particles, including pre-conditioning, followed by size-based discrimination for independent detection of fluorescent and non-fluorescent particles of different sizes. This is done by interactive tuning of a reduced set of parameters that can be checked with a robust, real-time quality control of the original video files. The software provides a concentration distribution of the particles in the transverse direction of the fluid flow. The software is a versatile tool for many microfluidic applications and does not require expertise in image analysis. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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