| Autor: |
de Gast, G. C., The, T. H., Ponds, E., Kallenberg, C. |
| Předmět: |
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| Zdroj: |
Clinical & Experimental Immunology; Dec1977, Vol. 30 Issue 3, p457-462, 8p |
| Abstrakt: |
The capacity of human peripheral blood lymphocytes to suppress DNA synthesis of other lymphocytes was studied in an assay consisting of two steps: firstly, activation by Con A during 24 hr followed by α-methylglucoside and mitomycin treatment; secondly, incubation of these Con A-activated 'suppressor' cells with autologous responder cells and stimulants, or incubation with allogeneic responder cells. The results were compared with cells similarly treated but not incubated with Con A. If α-methylglucoside treatment is omitted, stimulation of T and non-T cells occurs b) Con A bound to the Con A-activated cells. Con A is especially bound to non-T lymphocytes and even gives a T cell-independent proliferation of non-T cells without differentiation to plasma cells. With α-methylgkicoside treatment, 'suppressor' cells, activated by high Con A concentrations, are able to suppress DNA synthesis of autologous lymphocytes stimulated by allogeneic cells or soluble antigens to about 50%. In a one-way MLC, in which the cell suspension containing the suppressor cells is also used as a stimulator cell suspension, a similar suppression was observed. Suppression of DNA synthesis was correlated with suppression of proliferation without evidence of cytotoxicity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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