| Autor: |
Henle, Gertrude, Lennette, Evelyne T., Alspaugh, Margaret A., Henle, W. |
| Předmět: |
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| Zdroj: |
Clinical & Experimental Immunology; Jun1979, Vol. 36 Issue 3, p415-422, 8p |
| Abstrakt: |
Sera from twenty- eight patients with rheumatoid arthritis (RA) were titrated in indirect immunofluorescence tests for Epstein-Barr virus (EBV) specific antibodies. All had IgG antibodies to viral capsid antigen (VCA), 64% at titres ≧ 320, and 71% reacted also in tests for VGA-specific IgM antibodies at titres ranging from 20 to 640. The reactions observed in the IgM test were not due to VCA-specific IgM antibodies, however, but rather to rheumatoid factor (RF) usually an IgM antibody to the Fc regions of IgG. The titres recorded in the anti-VCA IgM test correlated significantly with the RF titres and both reactivities were abolished by adsorption onto IgG coated latex particles. In addition, they clearly depended upon the height of the IgG antibody titre to VGA, indicating that the more VGA-specific IgG molecules are present the more likely it is that RF will combine with them in sufficient quantity before or after their attachment to VGA-positive test cells so as to become detectable by the fluorescent antibodies to human IgM. Results com- parable in every aspect were obtained with those sera from patients with Hodgkin's disease, nasopharyngeal or cervical carcinomas which reacted in the anti-VGA IgM test. Sera from patients with infectious mononucleosis may also contain RF, but in such cases its removal by adsorption onto IgG-coated latex particles did not generally reduce the VGA-specific IgM antibody titre. Removal of RF from any of the sera studied did not affect the titres of VGA-specific IgG and, where applicable, IgA or heterophil antibody titres. These results re-emphasize the pitfall created by RF noted previously in tests for virus-specific IgM antibodies. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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