Stabilization of enzymes by dormancy autoinducers as a possible mechanism of resistance of resting microbial forms.

Autor: Kolpakov, A. I., Il’inskaya, O. N., Bespalov, M. M., KupriyanovaAshina, F. G., Gal’chenko, V. F., Kurganov, B. I., El’-Registan, G. I.
Zdroj: Microbiology (00262617); Mar2000, Vol. 69 Issue 2, p180-185, 6p
Abstrakt: Alkyl-substituted hydroxybenzenes (AHBs), autoinducers of microbial dormancy (ord1 factors), were found to stabilize the structure of protein macromolecules, making them metabolically less active and more resistant to stresses. In vitro experiments with theBacillus intermedius ribonuclease and chymotrypsin showed that the degree of the physical and chemical stability of these enzymes treated with AHBs depends on their concentration and incubation time. Experiments with RNase, which is capable of refolding, i.e., renaturation after heat denaturation, revealed that AHBs efficiently interact with both intact and denatured proteins. The data obtained allow the inference to be made thatd1 factors may play the role of natural chemical chaperons, blocking metabolism in dormant cells through the formation of catalytically inactive thermostable complexes with enzymes. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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