| Autor: |
Kyseľ, Matúš, Farkasová, Silvia, Štefúnová, Veronika, Zeleňáková, Lucia, Žiarovská, Jana |
| Předmět: |
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| Zdroj: |
Journal of Hygienic Engineering & Design; 2022, Vol. 41, p84-89, 6p |
| Abstrakt: |
Allergens of food resources possess great variability at the proteomic and genomic sequences and an actual part of research activities because of the raising number of food allergies worldwide. Actually, only a few in silico based allergen nucleotide sequences comparing study exist, in spite of the great variability in the individual allergen families and with the regard to understand biological background of naturally hypoand hyperallergenic plants. The aim of the study was to analyze the mutation potential of allergen sequences variability relevant for primer design allergens in different plant species in comparison to the amaranthus, one of the allergenic safe specie, that provide many nutrition benefits and is an alternative for cereals. Here, NCBI GenBank Nucleotide and WHO/IUIS Allergens Nomeclature Sub-Commitee databases data mining were acquired a nucleotide sequence records of pollen allergens as bioinformatic data for evaluation of the potential of Amarathus spp. allergen. Data mining was performed in the nucleotide sequence records of cross-reactive pollen allergens (oleosins and profilins) created automatically with a computational algorithm translated from protein sequence records as bioinformatic material. 7 sequences of oleosins and 7 sequences of profilins were manually modified and analysed. A universal 3-step method of data processing identified and quantified mutations with a help of terms hierarchy. Definitively, the least substituted base is G, the most frequent substitution is T > C, and the most reproducible substitutions are C > G and G > C (profilins) and A > T (oleosins). The final description of sequence relationship is based on oleosin and profilin substitutions of 4 different glances (substitutions Profiles, single substitutions, group substitutions and fused substitutions) seeming Sal k 4 > Ole e 2 > Bet av 2 > Ama v 1 > Vac f 1 > Cro s 1 > Che a 1 > Koc s 2 > Pro j 1 > Ama r 1, where Sequence Pro j 1 is the most related to the matrix sequence of Ama r 1. The rising amount of sequence records in GenBank database will influence a sequential likelihood of conclusions at the time, but substitution frequency, sequence order, and reproducibility should be kept stable. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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