| Autor: |
Jubair, Hani Hasan, Hadi, Zainab Jaber, Almohana, Ali M. |
| Předmět: |
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| Zdroj: |
Medico-Legal Update; Apr-Jun2020, Vol. 20 Issue 2, p461-466, 6p |
| Abstrakt: |
Background: The prevalence of the plasmid-borne colistin resistance gene mcr-1 in bacteria poses a potential threat to patient treatment, particularly when hospitalized spreading of this gene causes great concern as it can transmit between different bacteria species. The aims of this study were to investigate the presence of the mcr-1 gene among carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates from different clinical specimens and determine the clonal origin of strains carrying the mcr-1 gene using Multi-locus sequence typing (MLST) method. Method: In this study, 22 CRKP isolates from clinical specimens collected from the major four hospitals in Najaf/Iraq were examined. All isolates were identified by a standard biochemical test and confirmed by an automated Vitek®2 system. Antimicrobial susceptibility test was done on 12 antibiotics by the disk diffusion method. All isolates tested to detect the presence of the mcr-1 gene using the PCR method. Determine the sequence typing by MLST for all mcr-1-positive CRKP isolates. Results: Out of 147 K.pneumoniae, 22 carbapenem resistance isolates from different clinical specimens were detected. Antibiotic sensitivity test results revealed that all isolates (100 %) were resistant to ampicillin, Cefepime, Cefoxitin, Ceftazidime, and Ceftriaxone; however, most of the CRKP isolates (86.4 %) are sensitive to colistin. The mcr-1 gene was found in three (13.6%) of the 22 isolates of CRKP. These three isolates are resistant to all classes of antibiotics. The MLST results revealed that three mcr-1-positive CRKP isolates were related to three different sequence types: ST147, ST1, and ST11. Conclusion: The spread of CRKP isolates containing plasmid-borne mcr-1gene is worth our attention due to the consider of colistin as the last resort treatment against drug-resistant pathogens that increasingly identified in Najaf Hospitals/Iraq. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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