| Abstrakt: |
Alfalfa (Medicago sativa L.) is the most cultivated forage plant as a model in legumes. The growth and development of alfalfa root limited by salt stress. Growth regulator is an essential role of melatonin in plants, especially in root involved in stress tolerance. In this study, alfalfa root under in vitro culture was treated with salinity (150 and 200 mM NaCl) as well as different concentrations of melatonin (0.1, 10 and 15 µM). Treated plants by using melatonin increased compatible solutes such as glycine betaine, soluble carbohydrate and K+ under salt stress in alfalfa root. In contrast, melatonin decreased reactive oxygen species (ROS), hydrogen peroxide (H2O2), superoxide anion (O2−), malondialdehyde (MDA), electrolyte leakage (EL) and Na+ content in root treated with salinity. Interestingly, melatonin increased endogenous melatonin, indole-3-acetic acid (IAA), fresh and dry weight of the alfalfa root. According to our findings, all of the melatonin concentrations had a positive effect as a growth regulator but looking at the overall data 0.1 µM melatonin was the best candidate for increasing salt tolerance and reducing oxidative stress in alfalfa root. [ABSTRACT FROM AUTHOR] |
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