Genome-derived Microsatellite Markers in Lippia origanoides from Colombia.

Autor: Santamaría-Acevedo, Liliana, Levy, María Mercedes, Torres-Martínez, Lorena, Marchant, Sergio, Rondón-González, Fernando, Stashenko, Elena E., Levy, Morris, Fuentes, Jorge Luis
Zdroj: Journal of Herbs, Spices & Medicinal Plants; 2023, Vol. 29 Issue 2, p168-185, 18p
Abstrakt: Polymorphic microsatellite sequences were mined from genome sequences of two Lippia origanoides wild specimens from disjunct locations. Twenty-three primer sequences flanking the candidate markers were designed, and their usefulness for population genetic analysis was assessed in plants sampled from four municipalities in Colombia. A final set of 10 primer pairs was useful for diversity analyses, which indicated the existence of three genetic lineages in Colombia. Cross-amplification in five related Lippia species was validated. The findings supported the efficiency of in silico approaches in providing novel genetic resources for research in Lippia species. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index