| Autor: |
Bhat, Meghashyama P., Kumar, Raju Suresh, Almansour, Abdulrahman I., Arumugam, Natarajan, Dupadahalli, Kotresha, Rudrappa, Muthuraj, Shivapoojar Basavarajappa, Dhanyakumara, Sathyanarayana Swamy, Pallavi, Perumal, Karthikeyan, Nayaka, Sreenivasa |
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| Zdroj: |
Applied Nanoscience; Mar2023, Vol. 13 Issue 3, p2303-2314, 12p |
| Abstrakt: |
The current investigation was aimed to biologically synthesize silver nanoparticles utilizing Pyrostegia venusta leaf aqueous extract. The synthesized nanoparticles were characterized using UV–Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), X-ray diffraction (XRD), high-resolution-transmission electron microscopy (HR-TEM), zeta potential, and scanning electron microscopy (SEM) with energy-dispersive X-ray (EDX) analysis. The antimicrobial activity was done against bacterial and fungal pathogens like Enterococcus faecalis, Escherichia coli, Candida albicans and Candida glabrata and finally subjected to anticancer activity and cell apoptotic pathway assessment against African green monkey kidney (COS-7) cell line. The UV–Vis observation showed a maximum absorbance at 381 nm; the AFM data confirmed spherical shape and poly-dispersed nature of particles. Later, FTIR analysis revealed the patterning of IR spectral bands and TEM images determined the smallest size of biological AgNPs as 12.56 nm. Zeta potential showed a sharp peak at − 31.7 mv, whereas SEM/EDX depicted spherical shape and poly-dispersed nature of AgNPs with 42.95% presence of silver in sample. The antagonistic potential of synthesized AgNPs was well indicated by the zone of inhibition formed around the agar well against pathogens at varying concentrations. The AgNPs showed highest and lowest activity against E. coli and C. albicans, respectively. The AgNPs displayed significantly increased cytotoxic activity against COS-7 cells in vitro, with IC50 value of 50.48 µg/ml. The anticancer activity was also assessed using cell apoptosis; the results depicted the potential of AgNPs to kill COS-7 cells by differential staining to visualize both dead and live cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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