| Autor: |
Docema, Matheus Luís, Moraes, Tatiana de Souza, Attílio, Lísia Borges, Marques, João Paulo Rodrigues, Stipp, Liliane Cristina Liborio, Harakava, Ricardo, Freitas-Astúa, Juliana, Belasque Junior, José, Azevedo, Ricardo Antunes, Gaziola, Salete Aparecida, Mourão Filho, Francisco de Assis Alves |
| Zdroj: |
Plant Cell, Tissue & Organ Culture; Mar2023, Vol. 152 Issue 3, p471-489, 19p |
| Abstrakt: |
Currently, there are no resistant sweet orange cultivars to the citrus canker (cc) [Xanthomonas citri subsp. citri (Xcc)], and the production of transgenic plants resistant to cc can be an efficient solution in the search of improved cultivars. Several strategies can be applied in this scenario, including the expression of genes that code for antimicrobial peptides and resistance genes from Xcc-tolerant cultivars. We evaluated the reaction of 'Hamlin' sweet orange (Citrus sinensis L. Osbeck) transformed with a gene that codes for the D4E1 synthetic antimicrobial peptide, and 'Hamlin' sweet orange plants transformed with the gene that codes for a copper and zinc superoxide dismutase (csd1), isolated from Poncirus trifoliata, after inoculation with Xcc. Transgenic plants were propagated through grafting in 'Rangpur' lime (Citrus limonia Osbeck), and then inoculated with a Xcc suspension. Hydrogen peroxide production, activity of selected antioxidant enzymes and callose deposition were evaluated in the transgenic plants subjected to Xcc inoculation. The incidence and the severity of citrus canker were significantly reduced in several transgenic events, which also exhibited lower Xcc titers when compared with non-transgenic plants. Transgenic events which exhibited higher superoxide dismutase activities also showed increased accumulation of hydrogen peroxide in foliar tissues after Xcc inoculation. Epifluorescence microscopy analyses revealed a high callose deposition in the sieve tube elements of leaf tissues in some transgenic events. Callose accumulation is an important plant defense response to biotic stresses and, in csd1 transgenic lines, it was associated with lower Xcc populations. Key message: Transgenic sweet orange expressing genes by two different disease resistance strategies exhibited less susceptibility to citrus canker with potential to be further studied and integrated into mitigation of this disease. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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