Increasing the Radiosensitivity of MCF-7 Cancer Stem Cells Cultivated as Mammospheres to γ- and γ-Neutron Irradiation with Metformin.

Autor: Shuvatova, V. G., Kuvyrchenkova, A. P., Semochkina, Yu. P., Moskaleva, E. Yu.
Předmět:
Zdroj: Biology Bulletin; Dec2022, Vol. 49 Issue 12, p2314-2321, 8p
Abstrakt: Cancer stem cells (CSCs) are highly resistant to chemo- and radiotherapy. The aim of this work was to study the effect of metformin on the radiosensitivity of MCF-7 cells cultivated as mammospheres enriched with CSCs. One hour after the addition of metformin at a nontoxic concentration of 0.25 mmol/L, cells of mammospheres were exposed to γ-radiation at a dose of 2 Gy or reactor γ,n-radiation at a dose of 1 Gy. After 24 hours of cultivation, the drug was removed. Six days after irradiation, the total number of cells was calculated by a hemocytometer, then the percentage of CSCs with the CD44+/CD24–/low phenotype was determined, and the number of CSCs in the samples was calculated. The exposure to γ,n-radiation led to a significant decrease in both the total number of cells and CSCs compared with γ-radiation. The combined exposure to metformin and γ- or γ,n-radiation led to a significant decrease in both the total number of cells and CSCs compared with the exposure to γ-or γ,n-radiation only (p < 0.05). Thus, γ,n-radiation is more effective with respect to eliminating the MCF-7 CSCs than γ-radiation. Metformin increases the sensitivity of the MCF-7 CSCs to γ-and γ,n-radiation. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje