| Autor: |
Gerasimova, N. S., Akhtar, M. S., Studitskii, V. M. |
| Zdroj: |
Moscow University Biological Sciences Bulletin; Dec2022, Vol. 77 Issue 4, p216-222, 7p |
| Abstrakt: |
Previous studies have revealed the inhibitory effect of single-strand breaks in a non-template DNA strand (NT-SSB) on RNA polymerase (RNAP) transcription through the nucleosome. The observed effect was explained within the model of chromatin transcription mechanism with the formation of intranucleosomal DNA loops (i-loops), intermediates, in which the enzyme is locked in the DNA loop on the histone octamer. According to the model, NT-SSBs reduce the tension in the DNA structure caused by transcription, thus stabilizing DNA-histone interactions and inhibiting RNAP. In this work, the boundaries of such i-loops have been determined. It has been found that the formation of contacts between DNA and histones behind RNAP occurs at a distance of more than 17 base pairs from the active center of the enzyme. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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