| Abstrakt: |
In aquaculture, high mortality in crustacean larvae during the moulting process restricts the larviculture's productivity. This study aimed to determine the bioactive components in the methanolic extract of fiddlehead ferns, Diplazium esculentum, and their effects on the growth of Microbrachium rosenbergii postlarvae (PL). Hormonal analysis revealed a high level of 20-hydroxyecdysteroid (20E) hormone in both leaves and shoot parts (4733 pg/ml) with the presence of phenols, tannins, saponins, flavonoids, terpenoids, alkaloids, and steroids during the phytochemical screening. GC–MS analysis discovered the presence of thirty-two bioactive compounds, including 1-(2,6-dichlorobenzoyl)-2,5-dimethylpyrrole, decyl sulfide, beta-amyrin, and cyclotetrasiloxane, octamethyl- with the peak area of 12.60%, 10.81%, 10.46%, and 8.48%, respectively. During a pilot study, prawn PL were fed diets containing D. esculentum extracts, namely diet 1–diet 5, with concentrations of 0.5, 1.0, 2.0, 4.0, and 10.0 gkg−1, respectively. Results showed high survival in PL fed with diet 4 and diet 5 (75.56% and 77.22%, respectively; p = 0.049). Next, a 60-day feeding trial was conducted to determine the moulting and growth performance of PL fed with 0 gkg−1 (control), 4.0 gkg−1 (diet 4), and 10.0 gkg−1 (diet 5) fern extracts. Results showed that prawns fed with diet 5 significantly showed the highest moulting frequency (112 ± 1.32 moults) and moulting rate (2.04 ± 0.02 moults per prawn). The findings demonstrated that a higher concentration of 20E with the highest inclusion of D. esculentum in diets could have enhanced the moulting performance in M. rosenbergii. We suggest an experiment on nutrient-dose response to determining the optimum level for growth performance in M. rosenbergii. [ABSTRACT FROM AUTHOR] |
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