| Autor: |
Barjaktarović, Iva, Maletić, Jelena Stojčević, Vučinić, Nataša, Milutinović, Aleksandra, Grujičić, Maja, Čabarkapa, Velibor |
| Zdroj: |
Future Virology; Jan2023, Vol. 18 Issue 1, p31-38, 8p |
| Abstrakt: |
Aim: To evaluate the significance of E gene analysis in addition to N and RdRp genes of SARS-CoV-2, and to compare the specificity and sensitivity of targets. Materials & methods: We used two reverse transcription-PCR assays: one targeting N, E and RdRp and the other targeting N and RdRp genes and analyzed variation in threshold cycle (Ct) values. Results: Of the 155 samples, 70.32% tested positive: all three genes were detected in 45.87%, N and RdRp in 19.27% and only N in 34.86%. Patients negative for the E gene were tested after symptoms disappeared and Ct values were significantly higher. Conclusion: Samples negative for the E gene were potentially false positive and clinical conditions should be assessed while interpreting results. This study aimed to evaluate the significance of E gene detection in addition to the detection of N and RdRp genes of SARS-CoV-2 in diagnosing COVID-19. Patients negative for the E gene were tested after symptoms disappeared and it took significantly longer to detect signals in these samples. Samples negative for the E gene were potentially false positive and clinical data should be assessed while interpreting results. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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