| Autor: |
Goenaga‐Mafud, Loraine C., Gamez, Yordania Matos, Campos, Carolina P., Vollet‐Filho, Jose D., Inada, Natalia Mayumi, Kurachi, Cristina, Bagnato, Vanderlei Salvador |
| Zdroj: |
Journal of Biophotonics; Feb2023, Vol. 16 Issue 2, p1-11, 11p |
| Abstrakt: |
Organ transplantations have an increasing medical relevance. It is becoming a regular procedure with an increase in individuals waiting for organs. The increase in the number of discarded organs is mostly due to the donor's bacterial and/or viral infection. In this article, we are demonstrating the feasibility of reduction of the bacterial load in the kidney model by using Ultraviolet‐C (UV‐C) as a germicidal agent in circulating liquids. Using Staphylococcus aureus as a bacteria model, we were able to demonstrate that in less than 30 min of liquid circulation and associated to irradiation, the bacterial load of the perfusate Custodiol® HTK, histidine‐tryptophan‐ketoglutarate (solution with 5 log CFU ml−1), was fully eliminated. A modeling approach was created to verify the possibility of bacterial load decrease, when an organ (here, a renal experimental model) is present in the circuit, releasing a varied rate of microorganisms over time, while the solution is irradiated. Finally, we use an ex vivo model with a swine kidney, circulating in the preservation solution with a Lifeport® Kidney Transporter machine, to demonstrate that we can contaminate the organ and then promote the elimination of the microbiological load. The results show the feasibility of the technique. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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