Phenotypic study of CD4+ and CD8+ lymphocyte subsets in relation to cytomegalovirus carrier status and its correlate with pokeweed mitogen--induced B lymphocyte differentiation.

Autor:	Gratama, J. W., Langelaar, R. A., Oosterveer, M. A. P., van der Linden, J. A., Den Ouden-Noordermeer, A., Naipal, A. M. I. H., Visser, J. W. M., de Gast, G. C., Tanke, H. J.
Předmět:	LYMPHOCYTES B cells CELL differentiation T cells LEUCOCYTES BLOOD
Zdroj:	Clinical & Experimental Immunology; Aug1989, Vol. 77 Issue 2, p245-251, 7p
Abstrakt:	A characteristic of active cytomegalovirus (CMV) infection is its suppressive effect on in vitro assays of immune function. The expression of CD11b by the CD4⁺ and CD8⁺ lymphocytes allows the identification of subsets with distinct regulatory functions of pokeweed mitogen (PWM) induced B cell differentiation. In order to relate that result with our previous observations that CMV carriers have significantly increased numbers of CD4⁺, HNK1⁺ and CD8⁺, HNK1⁺ lymphocytes in their peripheral blood compared with non-carriers, we performed a three-colour flow cytometric analysis of the co-expression of CD11b and HNK1 by the CD4⁺and CD8⁺ lymphocytes obtained from 27 CMV carriers and 42 non-carriers. The differences between CMV carriers and non-carriers were significant for the CD4⁺, HNK1⁺ lymphocytes (median [5th and 95th percentiles], 59 [18 and 123 versus 24/7 and 73 per mm³, respectively; P&t;0.001) and CD8⁺, HNK1⁺ lymphocytes (105/28 and 259] versus 52 [23 and 139] per mm³; P<0.001), but not for the CD4⁺, CD11b⁺ lymphocytes (59 [18 and 135] versus 52 [17 and 104] per mm³) and the CD8⁺, CD11b⁺ lumphocytes (85 [34 and 293] versus 82 [21 and 248] per mm³). The CD4⁺, HNK1⁺ and CD8⁺, HNK1⁺ lymphocytes that were increased in CMV carriers compared with non-carriers included mostly CD11b^-, but also CD11b⁺ lymphocytes. After sorting CD4⁺ and CD8⁺ lymphocytes for four CMV carriers into HNK1⁺ and HNK1^- fractions, we analysed their regulatory functions on PWM-driven B cell differentiation. Helper function to PWM-driven B cell differentiation was exclusively associated with the CD4⁺, HNKI^- lymphocytes; the CD4⁺, HNK1⁺ generally did not show helper or suppressor activity in this assay. Both CD8⁺, HNK1⁺ and CD8⁺, HNK1^- lymphocytes showed suppressor activity. Thus, the HNK1 marker does not constitute a phenotypical correlate for suppressor cells of PWM-driven B- cell differentiation. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu