| Abstrakt: |
Introduction: Neuromuscular connection and synaptic transmission to all types of skeletal muscles may be affected by physical training. The purpose of this research is to investigate the effect of a period of high intensity interval training (HIIT) on the expression of calcitonin gene-related peptide (CGRP) in the soleus muscle of male rats. Materials and Methods: In this experimental study, 12 male Wistar rats weighing 200-220 grams were randomly placed into two training and control groups. HIIT training consisted of 6 to 12 repetitions of 2-minute interval training, with a one-minute rest between each repetition. Training was designed for 5 days a week and 6 weeks. The conditions of the control group were similar to the training group, but they did not exercise. 48 hours after the last training session, the rats were anesthetized by the standard method, sacrificed and the soleus muscle tissue of all the rats was extracted, then Western blotting was used to determine the level of CGRP expression. The independent t-test was used to check the significant difference between the control and HIIT groups. A significance level of p<0.05 was considered. Results: Statistical findings for CGRP receptor protein expression values showed that the implementation of 6 weeks of HIIT training protocol increased the expression of CGRP protein compared to the control group; This value was not statistically significant (p=0.078). Conclusion: Despite the increase in the expression of CGRP in the soleus muscle of rats, it seems that doing 6 weeks of HIIT training is not enough to cause a significant change in the expression of the CGRP gene in the soleus muscle of rats, and it should be Longer protocols should be used. [ABSTRACT FROM AUTHOR] |
