| Autor: |
Paul, Sayan, Arumugaperumal, Arun, Balakrishnan, Subburathinam, Jebasingh, Emmanuel Joshua, Thangapandi, Sathiya Balasingh, Devi, Huidrom Sarjubala, Johnson, Thang, Maisnam, Shyam, Syamala, Sandhya Soman, Ramamoorthy, Sivakumar, Karthikeyan, Raman, Subburaman, Chandramohan, Alaguponniah, Sathyalakshmi, Krishna, Deepa Velayudhan, Nallaperumal, Krishnan, Singh, Salam Shantikumar, Azhaguchamy, Muthukumaran, Jeyaprakash, Rajendhran, Ramaiyer, Varatharajan, Ashmi, John |
| Zdroj: |
Journal of Experimental Zoology India; Jan2023, Vol. 26 Issue 1, p1219-1240, 22p |
| Abstrakt: |
The cabbage butterfly, Pieris brassicae (L.) is an economically important cosmopolitan insect, that causes appreciable crop loss in cabbage, cauliflower and other cruciferous crops, especially during winter and early spring in India. To reduce its pest density, a granulovirus infecting Pieris brassicae (PibrGV) was found to be a promising eco-friendly pathogen in the present study. Our report describes the pathogenicity, structural details, genome sequence, and characterization of the PibrGV Manipur isolate. The median lethal concentration and survival time were calculated for the third instar larvae of P. brassicae and the bioassay revealed that LC50 and LT50 of the virus were found to be 4.8 x 104 OBs/ml and 120 hours, respectively. Post-purification of occlusion bodies, the viral DNA was extracted and subjected to genome sequencing and annotation. There were 128 Glimmer-predicted ORFs in the 106,469 bp long viral genome characterized by 33.7% GC content. The study further indicated that a total of 14 genes were involved in replication, 10 genes were associated with transcription and 24 genes were related to structure formation, respectively. The above database will help in understanding the molecular mechanisms and genetic makeup of the virus and assist in pest management strategies through recombinant DNA technology. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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