Altered expression of NLRP3 inflammasome in pediatric patients with primary immune thrombocytopenia and its relation to disease activity.

Autor: Mohamed, Yasmen A., Morgan, Dalia S., Abd-Elkareem, Rehab M., Moneam, Nora A.
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Zdroj: Comparative Clinical Pathology; Feb2023, Vol. 32 Issue 1, p37-41, 5p
Abstrakt: Immune thrombocytopenia (ITP) is an acquired autoimmune disease that is characterized by dysregulation of T cell-mediated autoimmunity. NLRP3 (NOD-like receptor proteins) is one of the largest inflammasomes. It is crucial for the control of adaptive immunological response, particularly T cell responsiveness. Evaluation of the NLRP3 inflammasome's contribution to the pathogenesis of ITP and its relationship to disease activity and time of remission are the main objectives. There were 50 matched healthy controls and 60 pediatric ITP patients (20 with active ITP and 40 in remission). ITP patients in remission were divided into 3 subgroups (14 newly diagnosed, 6 persistent, and 20 chronic ITP). For measurement of NLRP3 mRNA levels by real-time quantitative PCR, peripheral blood mononuclear cells from patients and controls were extracted for RNA isolation. There was a significant negative correlation between platelet counts and NLRP3 expression. Additionally, a significantly higher level of NLRP3 was detected in patients with active ITP compared to the control (p value <.001), while NLRP3 in ITP patients in remission was significantly lower than in active ITP patients (p value <.001) and higher than in control but with no significant value. Among the subgroups of patients in remission, the expression level was significantly higher in patients with chronic ITP than newly diagnosed (p value <.033). Increased NLRP3 expression was linked to ITP pathogenesis as well as to disease activity and time of remission. NLRP3 may be a new approach in the follow-up, prevention, and treatment of ITP. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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