| Autor: |
Hussin, Ainulkhir, Md Nor, Norefrina Shafinaz, Bahtiar, Adibah Ahamad, Yamin, Bohari Mohd, Ibrahim, Nazlina |
| Předmět: |
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| Zdroj: |
Malaysian Applied Biology; 2022, Vol. 51 Issue 5, p261-269, 9p |
| Abstrakt: |
A major problem faced in treatment of virus infection is the presence of (Human Herpes virus type 1, HHV-1) antiviral drug resistant viruses i.e. acyclovir. Thus, search for drugs with the capability to encounter acyclovir-resistant HHV-1 is urgently needed. This study is aimed to configure the goniothalamin (GTN) isolated from Goniothalamus umbrosus followed by identifying the in vitro ability to inhibit acyclovir-resistant HHV-1 mutants. Crystals developed following extraction from the root and bark of G. umbrosus was confirmed by GC-MS, FTIR and NMR as goniothalamin. The J coupling peak calculation confirms that the active compound was in a cis-configuration. Prior to the antiviral activity determination, the cytotoxic concentration of GTN that killed 50% of the vero cell population (CC50) was determined with a CC50 value of 8.747 μg/mL. GTN concentration lower than the CC50 value was used in the determination of antiviral activity. Eleven HHV-1 mutant isolates were tested in the post-treatment assay to determine the antiviral activity. The effective concentration that reduces 50% of viral plaque formation (EC50) ranged from 0.47 μg/mL (2.35 μM) to 1.42 μg/mL (6.42 μM). The selective index (SI) ratio that determines the effectiveness of antiviral activity to cytotoxicity is between 6 to 17 which indicates that GTN has good potential to act as an antiviral agent. In conclusion, GTN in cis configuration has the ability to inhibit acyclovir-resistant mutant isolates. This potential is important to encounter the current problem in transmission of ACV-resistant mutants especially with thymidin kinase or DNA polymerase mutations. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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