| Abstrakt: |
Increasing Indonesia's native chicken population is needed to make this kind of chicken as a food source. The way to make the population increase is by using artificial insemination. Artificial insemination needs sperm dilution to increase the amount of semen that can be used. In sperm dilution need various kind of materials to maintain the semen quality also to extend life period of the semen itself. Spermatozoa could not live longer without supporting elements such as glucose, sorbitol, and organic ions like Na, Cl, K and Mg. These elements support sperm live ability in semen, and it is comfort place for microorganism to grow, then it can contaminate the spermatozoa. Alternative components can be alternative to reduce the microorganism growth. Betel leaves contain eugenol that can used as antibacterial on semen's diluents. The research was objected to find the best level of addition betel leaf extract on physiological NaCl to be added on native chicken semen as diluents on 5 0C. Method that used was experimental method with complete randomized design of a split – plot pattern consists of 4 treatment (0%, 1%, 2,5%, and 4%) as main plot and 5 storage times (0 hours, 2 hours, 4 hours, 6 hours. and 8 hours) as sub plot which was repeated 5 times. The results were analyzed by analysis of variance and followed by the DMRT test. The variables tested were motility, viability, abnormality, and pH. Addition of betel leaf extract doesn't have any significant effect (P>0,05) on every variable tested. The storage time had significant effect (P<0,05) on motility and pH. On the other hand, there's no significant effect on abnormality and viability. [ABSTRACT FROM AUTHOR] |
