| Autor: |
Niedziela, Agnieszka, Wasiak, Marzena, Wojciechowska, Marzena, Orłowska, Renata, Dynkowska, Wioletta Monika, Bany, Sławomir, Bednarek, Piotr Tomasz |
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| Zdroj: |
Crop Science; Jan/Feb2023, Vol. 63 Issue 1, p43-54, 12p |
| Abstrakt: |
The Triticum timopheevii Zhuk. (Tt) cytoplasm is the most exploited cytoplasmic male sterility (CMS) type used in triticale in commercial seed production of hybrid varieties. The present study established a recombinant inbred line (RIL) mapping population to identify genetic loci controlling fertility restoration in triticale with CMS Tt. The trait was evaluated via phenotyping the BC1F1 materials originating from a backcross of maternal line MS 112(15)‐2‐1 (on CMS Tt) and RILs. A genetic map spanning 2293 cM over 21 linkage groups was constructed using 1,170 single nucleotide polymorphisms and silico diversity array technology (DArT) markers generated through DArTseq genotyping. Two quantitative trait loci (QTL) controlling fertility restoration were localized at the 4RL chromosome. They were stable during two vegetative seasons and, depending on the year, explained 17.8 to 37.5 (QRft‐4R.1) and 17.8 to 27.1% (QRft‐4R.2) of phenotypic variance. Bioinformatic analysis was performed to predict genes potentially responsible for the trait. Four markers linked to the trait were successfully converted into a polymerase chain reaction‐based assay to select the pollen fertility QTLs easily. The exact position of QTLs detected previously in the rye and here in the triticale suggests that the same loci might control the trait. Core Ideas: We developed RIL mapping populations to detect QTLs controlling CMS Tt system in triticale.We constructed a high‐density genetic map using SNP and silico DArT markers.We identified SNP and silico DArT markers linked to and/or associated with the trait.We converted selected markers into PCR‐based assay for easy selection of the pollen fertility QTLs.We indicated genes potentially responsible for fertility restoration in triticale with CMS Tt. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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