| Autor: |
da Costa, Vanessa Duarte, Santos, Alanna Calheiros, da Silva, Lucas Lima, Wiggers, Wilian Jean, Ivantes, Claudia Alexandra Pontes, Lima, Danielle Malta, Colares, Jeová Keny Baima, Dallacqua, Deusilene Souza Vieira, Motta-Castro, Ana Rita Coimbra, Dávila, Alberto Martín Rivera, Teles, Sheila Araujo, Carneiro, Megmar Aparecida dos Santos, Caetano, Karlla Antonieta Amorim, Anunciação, Fernando Antonio Costa, de Paula, Vanessa Salete, Villar, Livia Melo |
| Předmět: |
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| Zdroj: |
Diagnostics (2075-4418); Jan2023, Vol. 13 Issue 2, p210, 13p |
| Abstrakt: |
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a rapid method that can replace RT-qPCR. A simple molecular assay for SARS-CoV-2 RNA detection in gold-standard diagnosis through swabs and alternative specimens such as saliva could be helpful in promoting genomic surveillance. A multicenter study was conducted to evaluate the RT-LAMP assay method as an alternative for the molecular detection of SARS-CoV-2 lineages in swab and saliva samples. A total of 350 swabs from individuals with (n = 276) or without (n = 74) COVID-19 tested by RT-qPCR were collected. Paired saliva was also collected from 90 individuals who had SARS-CoV-2 RNA that was detectable (n = 30) or undetectable (n = 60) via RT-qPCR. For the RT-LAMP methodology, six primers were used for ORF1 gene amplification. As for SARS-CoV-2 genotyping, 39 swabs had the whole genome sequenced by MinION. The sensitivity of RT-LAMP to the swab was 90.2%. For the swab samples with Ct ≤ 30, the sensitivity improved by 96%. Considering saliva with Ct ≤ 30 in RT-qPCR testing, the RT-LAMP sensitivity was 100%. The RT-LAMP specificity was 100% for both the swab and saliva samples. This RT-LAMP assay was capable of detecting all the SARS-CoV-2 lineages circulating in the Brazilian swab samples. The RT-LAMP method has significant potential for use in clinical routines since it was capable of detecting SARS-CoV-2 RNA in swab and saliva samples. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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