| Abstrakt: |
Recovery of proliferative stem cells from postmortem tissues is an important area of research due to its potential applications in cellular therapies in human and veterinary medicine, and preservation of germplasm for future revival of lost genetics. The objective of this study was to assess the postmortem time interval (PMI) within which proliferative cells could be recovered from the tissues of newborn goat kids after their death. Skin explants (3-4 mm2; n = 20) were cultured in Dulbecco's modified eagle medium (DMEM) supplemented with 10% Fetal bovine serum (FBS) after 0, 7, 14, 21, and 28 days of postmortem (dpm) storage at 4°C. Outgrowth of cells was seen only up to 14 days of PMI in all (100%) of explants but not beyond that. Outgrowth started on day 3, 5, and 8 in 0-dpm, 7-dpm, and 14-dpm time intervals, respectively. The level of confluence was inversely corelated with increasing PMI. The average number of cells recovered from each explant was 26.11 x 104, 7.10 x 104, and 2.40 x 104 cells/mL for 0-dpm, 7-dpm, and 14-dpm cultures, respectively. Subcultures of these primary cells, when plated in equal numbers, showed comparable growth profile to that of fresh tissue derived cell populations. This study showed that postmortem tissues retained proliferative cells for much longer time than it was previously thought, and thus, could be preserved even after several days of animal death to recover the lost genetics in future. [ABSTRACT FROM AUTHOR] |
