| Abstrakt: |
Liangfenghua chicken is a meat variety selected by hybridization of local yellow feather chickens in China and is the first new poultry variety approved by Chinese government. However, there have been no relevant reports on the structure of intestinal microbial bacteria in Liangfenghua chicken. The purpose of this research was to study the composition and function of intestinal microflora in different parts of Liangfenghua chicken by 16SrDNA highthroughput sequencing technology. The results showed that the flora of the cecum was all clustered together, and the flora between the ileum, jejunum, and duodenum were crossed. The cecal samples clustered more concentratedly, and the microbial composition of the samples within the group was more uniform. At the phylum level, microorganisms in the four intestinal sections involved a total of 19 phyla, mainly Firmicutes and Bacteroidetes. The relative abundance of Firmicutes in duodenum, jejunum, and ileum was more than 84%, which was significantly higher than that in cecum (41.05%), while the relative abundance of Bacteroidetes, synergized and unclassified in duodenum, jejunum, and ileum was close to 0%, which was significantly lower than that in cecum (P < 0.05). At the genus level, the microorganisms in the four intestinal sections involved 229 genera, 40 genera with high relative abundance, of which 20 had significant differences between groups. The relative abundance of Lactobacillus in duodenum (group D), ileum (group I) and jejunum (group J) was more than 80%, which was significantly higher than that in cecum (group C) (3.8%). The relative abundance of Bacillus in group I was significantly higher than that in groups J and C (P < 0.05), which was also higher than that in group D, but without significant difference. The relative abundance of 18 genera including Bacteroides, unclassified, Alistipes, and Barnesiella was significantly higher in group C than in groups D, I, and J (P < 0.05). There were significant differences in microbial composition in different intestinal sections of Liangfenghua chicken, and the microbial diversity in cecum was higher than that in duodenum, jejunum, and ileum. The dominant bacteria in the upper intestine were Firmicutes and Lactobacillus, and the relative abundance of Bacillus jejuni was higher than that in other sections. The dominant bacteria in cecum were Firmicutes and Bacteroides, and the dominant bacteria were rumen cocci, Clostridium, Bacteroides, which had digestive function, produce short chain fatty acids, and were conducive to the immune system. This study laid a foundation for the feed formulation and feeding management of Liangfenghua chicken in the replacement period. [ABSTRACT FROM AUTHOR] |
