| Abstrakt: |
[Objectives] The paper was to establish the quality standard of Yi herb medicine Millettia dielsiana. [Methods] Yi herb medicine M. dielsiana was qualitatively identified by microscopic identification and thin layer identification. The content of total isoflavones was determined by UV-Vis spectrophotometry, and the contents of moisture, total ash, acid-insoluble ash and extract were checked according to the method introduced in Chinese Pharmacopoeia (2020 edition). [Results] The microscopic identification features, including stone cells, fiber bundles, calcium oxalate square crystals, starch granules, pigment blocks, ducts, etc., were distinct. A TLC method for identification of M. dielsiana was established. The contents of water, total ash, acid-insoluble ash and extract should be no more than 13%, no more than 7%, no more than 0.5% and no less than 6% in 10 batches of samples, respectively. The total isoflavones of M. dielsiana based on genistein (C15H10O5) should be no less than 0.25%. The linear relationship of genistein was good in the range of 0.002 - 0.007 mg/mL (R² = 0.999 6) ; the average recovery was 97.01 % and the RSD was 2. 62%. [Conclusions] The method is simple, accurate and well-reproducible, and can be used for the quality control of Yi herb medicine M. dielsiana. [ABSTRACT FROM AUTHOR] |
