| Autor: |
Hu, Ying, Zhang, Wei, Shen, Xiufen, Qu, Qiaoli, Li, Xiao, Chen, Rucai, Wang, Zhuo, Ma, Run, Xiong, Zaikun, Wang, Yuming, Wang, Pengfei |
| Předmět: |
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| Zdroj: |
Infection & Drug Resistance; Dec2022, Vol. 15, p7431-7447, 17p |
| Abstrakt: |
Introduction: Carbapenem-resistant Klebsiella pneumoniae (CRKP) has been widespread in coastal cities of eastern China since 2009. However, how CRKP spreads and evolves in southwest China is unclear. Aim: We investigated the genetic characteristics and dissemination mechanisms of carbapenemase genes in forty-one non-repetitive CRKP isolates collected from a southwest hospital, Kunming, Yunnan, during 2010– 2013. Methodology: Drug susceptibilities were analyzed by using VITEK 2 compact system. Genetic relationships were ascertained based on multilocus sequence typing (MLST) and Pulsed-field gel electrophoresis (PFGE) analysis. Genetic backgrounds of blaKPC-2 and blaNDM-1 were revealed by DNA walking and high-throughput sequencing. Results: All isolates were highly resistant to common antibiotics except for tigecycline. In total, 34 blaKPC-2, 3 blaNDM-1, 1 blaIMP-4 and 3 blaIMP-26 genes were identified and KP67 plasmid 1 co-harbored blaNDM-1 and blaIMP-26. Five sequence types, namely ST11, ST290, ST340, ST395 and ST437, were recognized by MLST. Surprisingly, blaKPC-2 was only detected in ST11 strains. We described a clonal dissemination of fosA3-positive IncR-IncF33:A-:B- multireplicon plasmid carrying the gene cassettes IS 26-ΔTn 3-ISKpn27-blaKPC-2-ΔISKpn6-korC-klcA-ΔrepB-Tn 1721 in all ST11 isolates. Three blaNDM-1 positive isolates belonged to three different ST types and their blaNDM-1 genetic backgrounds were also distinct. Interestingly, the flanking regions of blaNDM-1 in KP67 and KP72 were duplicated into one to five copies in a form of tandem repeat by the transposition of IS 91 like element. The blaNDM-1 of KP82 was carried on a common IncX3 plasmid. Conclusion: This study described the early epidemiological characteristics of blaKPC-2/blaNDM-1-carrying CRKP, and reported a new tandem repeat pattern of blaNDM-1 cluster in Yunnan. These findings extend our knowledge on the carbapenemase gene evolutions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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