| Autor: |
Hemmaphan, Sirirak, Bordeerat, Narisa K. |
| Předmět: |
|
| Zdroj: |
EnvironmentAsia; Sep2022, Vol. 15 Issue 3, p123-132, 10p |
| Abstrakt: |
Lead (Pb) is a common environmental pollutant with potential genotoxic properties. However, the molecular mechanisms underlying Pb genotoxicity related to inhibition of DNA repairs are not fully elucidated. This study aimed to investigate the impact of Pb on DNA repair genes and proteins expression. The acute and chronic Pb-exposed human renal proximal tubular cell models (RPTEC/TERT1 cells) were established. The cell viability, cellular Pb level, changes in expression of DNA repair genes and proteins (XRCC1, hOGG1, and ERCC1) were measured. The cell viability assay showed a significant decrease in cell viability in a dose-dependent manner. The AAS analysis showed cellular Pb levels indicating bioaccumulation of Pb in treated cells. RT-PCR and Western blot results demonstrated that the mRNA and protein levels of XRCC1 significantly decreased after acute and chronic exposure. hOGG1 and ERCC1 showed a significant decrease in mRNA level after acute exposure, whereas no significant change in their protein levels after both acute and chronic exposure was observed. In conclusion, our study provides the first evidence to show that acute and chronic Pb exposure results in altered expression of DNA repair genes and proteins and support the possible genotoxic properties of Pb to inhibit the DNA repairs. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
