| Autor: |
Reinders, Lars M. H., Klassen, Martin D., Endres, Patrick, Krumm, Andrea, Jaeger, Martin, Schmidt, Torsten C., Teutenberg, Thorsten |
| Zdroj: |
Chromatographia; Jan2023, Vol. 86 Issue 1, p79-85, 7p |
| Abstrakt: |
A monoclonal antibody's (mAb) glycosylation pattern has a significant impact on its binding affinity to the FcγRIIIa receptor and thus on antibody-dependent cell-mediated cytotoxicity. However, to analyze the relation in cell cultures, complex sample preparation is required. In this study, we demonstrate the analytical capability of a two-dimensional liquid chromatography–mass spectrometry (2D-HPLC–HRMS) method to analyze the affinity of mAbs to the FcγRIIIa receptor while simultaneously characterizing the glycoforms involved. Results were comparable in purified samples and cell culture-free supernatants. This was demonstrated with the monoclonal antibodies adalimumab, pertuzumab, ustekinumab, tocilizumab and omalizumab. Our method hence renders extensive purification of mAb samples superfluous. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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