| Abstrakt: |
Objective: The pharmacokinetics and relative bioavailability of Jiaotai pill self-microemulsion were evaluated by investigating the blood concentration of Berberine, Coptisine, Palmatine and Jatrorrhizine in insomnia rats. Methods: Insomnia rat model was established by intraperitoneal injection of p-chlorophenylalanine (PCPA). The model rats were given Jiaotai pill selfmicroemulsion and Jiaotai pill suspension. The contents of Berberine, Coptisine, Palmatine and Jatrorrhizine in plasma at different times after administration were determined by UPLCMS/MS, and calculate pharmacokinetic parameters. Results: Under the set chromatographic conditions, the linear relationship of the four components was good, and the precision, accuracy and stability meet the requirements of biological samples. After intragastric administration of Jiaotai pill self-microemulsion, The Cmax of Berberine, Coptisine, Palmatine and Jatrorrhizine were (412.68±28.45), (68.65±3.92), (34.06±3.13), (40.60±1.22) ng/mL, and AUC0.4 were (672.70±72.55), (146.04±25.01), (71.49±18.67), (72.25±9.54) ng-mL-1, respectively. Compared with Jiaotai pill suspension, the Cmax, AUC0-4 and AUC0-/of the four components in insomnia rats were significantly increased (P<0.01). Conclusion: Jiaotai pill self-microemulsionl can promote the absorption of effective components in insomnia rats and improve its bioavailability. [ABSTRACT FROM AUTHOR] |
